Epithelial tumour cell detection and the unsolved problems of nested RT-PCR: a new sensitive one step method without false positive results

development of a new- fast and sensitive one step/one tube nested rt-pcr method in a closed system by double-joint primer for detecting hcv

objective: polymerase chain reaction (pcr) is one of the most important progress in the field of molecular biology and diagnosis. despite simplicity in concept, the reaction needs complex interaction between target sequence, primers, dntps and dna polymerase for a successful amplification and diagnosis. for the detection of rna viruses highly sensetive and specific technique is required. hence ...

the use of a sensitive rt-nested pcr method for detection of hepatitis c virus

objective: hepatitis c virus is the major cause of viral hepatitis and its diagnosis in suspected specimens is of great importance. the risk of transfusion- transmitted virus infection is primarily the result of failure in serological screening tests to detect recently infected donors in the pre-seroconversion window period of infection. therefore, sensitive and accurate diagnosis of hcv prior ...

A simple method for elimination of false positive results in RT-PCR.

Discrimination between the amplification of mRNA and contaminating genomic DNA is a common problem when performing a reverse transcriptase-polymerase chain reaction (RT-PCR). Even after treatment of the samples with DNAse, it is possible that negative controls (samples in which no reverse transcriptase was added) will give positive results. This indicates that there was amplification of DNA, wh...

Estimation of relative mRNA content by filter hybridization to a polyuridylic probe.

1.Battaglia, M., P. Pedrazzoli, B. Palermo, A. Lanza, F. Bertolini, N. Gibelli, G.A. Da Prada, A. Zambelli et al. 1998. Epithelial tumour cell detection and the unsolved problems of nested RT-PCR: a new sensitive one step method without false positive results. Bone Marrow Transplant 22:693-698. 2.Collins, C., J.M. Rommens, D. Kowbel, T. Godfrey, M. Tanner, S.I. Hwang, D. Polikoff, G. Nonet et a...

One-Step Reverse Transcription PCR (RT-PCR). Fatemeh Mehrpooyan*